Rat OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit

Product Specifications

Abbreviation

OxLDL

Cross Reactivity

This kit recognizes Rat OxLDL in samples. No significant cross-reactivity or interference between Rat OxLDL and analogues was observed

Target

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OxLDL. You can calculate the concentration of Rat OxLDL in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.47-30 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.28 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.liebertpub.com/doi/abs/10.1089/ars.2022.0065

Citation 02

https://www.mdpi.com/1304184

Citation 03

https://www.mdpi.com/2072-6643/13/5/1569

Citation 04

https://www.mdpi.com/1424-8247/14/4/341

Citation 05

https://www.hindawi.com/journals/omcl/2019/1965364/abs/

Citation 06

https://www.hindawi.com/journals/omcl/2016/8379105/abs/

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4524384/

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.6369

Citation 09

https://www.hindawi.com/journals/ecam/2022/8882993/

Citation 10

https://www.tandfonline.com/doi/abs/10.3402/fnr.v59.29046

Citation 11

https://synapse.koreamed.org/articles/1128961

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat OxLDL concentrations in serum, plasma and other biological fluids.

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