Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit

Product Specifications

Abbreviation

MCP-1

UniProt

P14844

Cross Reactivity

This kit recognizes Rat MCP-1 in samples. No significant cross-reactivity or interference between Rat MCP-1 and analogues was observed

Target

CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1007/s42765-022-00224-w

Citation 02

https://link.springer.com/article/10.1186/s12951-022-01636-x

Citation 03

https://www.mdpi.com/2072-6643/14/15/3247

Citation 04

https://www.sciencedirect.com/science/article/pii/S0141813021010412

Citation 05

https://www.sciencedirect.com/science/article/pii/S0753332221007034

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8579055/

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29453

Citation 08

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03403k

Citation 09

https://europepmc.org/article/med/35991671?javascript_support=no

Citation 10

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.201902943R

Citation 11

https://www.sciencedirect.com/science/article/pii/S1382668921000430

Citation 12

https://www.sciencedirect.com/science/article/pii/S0269749117348984

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat MCP-1 concentrations in serum, plasma and other biological fluids.

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