Rat LEP (Leptin) ELISA Kit

Product Specifications

Abbreviation

LEP

UniProt

P50596

Cross Reactivity

This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed

Target

LEPD; OB; OBS; Obesity Homolog

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/989394

Citation 02

https://molmed.biomedcentral.com/articles/10.1186/s10020-022-00589-z

Citation 03

https://europepmc.org/article/pmc/pmc9189289?javascript_support=no

Citation 04

https://pubmed.ncbi.nlm.nih.gov/34305591/

Citation 05

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03403k

Citation 06

https://www.sciencedirect.com/science/article/pii/S0960076022001303

Citation 07

https://www.sciencedirect.com/science/article/pii/S0899900722003264

Citation 08

https://www.sciencedirect.com/science/article/pii/S0955286320304903

Citation 09

https://www.sciencedirect.com/science/article/pii/S1319016423000105

Citation 10

https://www.sciencedirect.com/science/article/pii/S0306453022002037

Citation 11

https://journals.physiology.org/doi/abs/10.1152/ajpendo.00093.2021

Citation 12

https://www.nature.com/articles/s41598-022-09541-x

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat LEP concentrations in serum, plasma and other biological fluids.

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