Rat NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit

Product Specifications

Abbreviation

NOS2; iNOS

UniProt

Q06518

Cross Reactivity

This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed

Target

NOS2A; HEP-NOS; I-NOS

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS2/iNOS. You can calculate the concentration of Rat NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332218335200

Citation 02

https://www.mdpi.com/2076-3921/11/2/255

Citation 03

https://www.sciencedirect.com/science/article/pii/S0141813022007279

Citation 04

https://www.mdpi.com/1103930

Citation 05

https://cmjournal.biomedcentral.com/articles/10.1186/s13020-021-00562-8

Citation 06

https://www.frontiersin.org/articles/10.3389/fphar.2021.741623/full

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253514/

Citation 08

https://www.mdpi.com/1871692

Citation 09

https://www.sciencedirect.com/science/article/pii/S0753332220309744

Citation 10

https://journals.physiology.org/doi/abs/10.1152/ajpendo.00093.2021

Citation 11

https://www.nature.com/articles/s41598-022-09380-w

Citation 12

https://rep.bioscientifica.com/view/journals/rep/160/6/REP-20-0381.xml

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat NOS2/iNOS concentrations in serum, plasma and other biological fluids.

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