Rat HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Product Specifications

Abbreviation

HIF-1α

UniProt

O35800

Cross Reactivity

This kit recognizes Rat HIF-1α in samples. No significant cross-reactivity or interference between Rat HIF-1α and analogues was observed

Target

HIF1A; HIF-1A; MOP1; PASD8; bHLHe78; basic helix-loop-helix transcription factor

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/2076-3921/11/11/2152

Citation 02

https://www.sciencedirect.com/science/article/pii/S0014299923000092

Citation 03

https://journals.sagepub.com/doi/abs/10.1177/09636897221102898

Citation 04

https://www.tandfonline.com/doi/abs/10.2147/DDDT.S391606

Citation 05

https://www.sciencedirect.com/science/article/pii/S0378874121010308

Citation 06

https://www.sciencedirect.com/science/article/pii/S1567576920337723

Citation 07

https://link.springer.com/article/10.1007/s10571-020-00981-5

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.14397

Citation 09

https://journals.physiology.org/doi/abs/10.1152/ajprenal.00625.2020

Citation 10

https://www.spandidos-publications.com/10.3892/mmr.2022.12689

Citation 11

https://link.springer.com/content/pdf/10.1007/s00210-019-01765-5.pdf

Citation 12

https://www.ajol.info/index.php/tjpr/article/view/201646

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat HIF-1α concentrations in serum, plasma and other biological fluids.

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