Rat CRP (C-Reactive Protein) ELISA Kit

Product Specifications

Abbreviation

CRP

UniProt

P48199

Cross Reactivity

This kit recognizes Rat CRP in samples. No significant cross-reactivity or interference between Rat CRP and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CRP. You can calculate the concentration of Rat CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12168

Citation 02

https://www.sciencedirect.com/science/article/pii/S075333222300118X

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8376584/

Citation 04

https://www.dovepress.com/gastroprotective-effect-of-azilsartan-through-ameliorating-oxidative-s-peer-reviewed-fulltext-article-JIR

Citation 05

https://www.mdpi.com/2103520

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332221012531

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332222001664

Citation 08

https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202001133

Citation 09

https://www.sciencedirect.com/science/article/pii/S0378874122009783

Citation 10

https://www.nature.com/articles/s41598-022-18326-1

Citation 11

https://www.mdpi.com/2072-6643/12/11/3511

Citation 12

https://www.mdpi.com/article/10.3390/ijerph19137921

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CRP concentrations in serum, plasma and other biological fluids.

Available Sizes

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