Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit

Product Specifications

Abbreviation

HMGB-1

UniProt

P63159

Cross Reactivity

This kit recognizes Rat HMGB-1 in samples. No significant cross-reactivity or interference between Rat HMGB-1 and analogues was observed

Target

HMGB1; HMG1; HMG3; SBP-1; Amphoterin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Epigenetics and Nuclear Signaling

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HMGB-1. You can calculate the concentration of Rat HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/article/10.3390/cells12010185

Citation 02

https://www.sciencedirect.com/science/article/pii/S0024320522006397

Citation 03

https://www.mdpi.com/1422-0067/23/20/12615

Citation 04

https://www.mdpi.com/1424-8247/15/7/852

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.17062

Citation 06

https://www.hindawi.com/journals/mi/2020/1320278/

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/adb.12365

Citation 08

https://www.nature.com/articles/s41598-021-94928-5

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576104/

Citation 10

https://www.sciencedirect.com/science/article/pii/S0753332219306766

Citation 11

https://www.sciencedirect.com/science/article/pii/S0300483X15001146

Citation 12

https://www.physiology.org/doi/abs/10.1152/ajpcell.00148.2018

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat HMGB-1 concentrations in serum, plasma and other biological fluids.

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