Rat FSH (Follicle Stimulating Hormone) ELISA Kit

Product Specifications

Abbreviation

FSH

UniProt

P11962 (FSHA) &P18427 (FSHB)

Cross Reactivity

This kit recognizes Rat FSH in samples. No significant cross-reactivity or interference between Rat FSH and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FSH. You can calculate the concentration of Rat FSH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.13-200 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.88 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0269749122020589

Citation 02

https://www.sciencedirect.com/science/article/pii/S0144861722002089

Citation 03

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-03056-y

Citation 04

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 05

https://biolres.biomedcentral.com/articles/10.1186/s40659-022-00401-6

Citation 06

https://www.mdpi.com/2076-3921/11/10/1879

Citation 07

https://www.sciencedirect.com/science/article/pii/S0891584922000764

Citation 08

https://www.sciencedirect.com/science/article/pii/S014765132200656X

Citation 09

https://www.hindawi.com/journals/omcl/2022/2113293/

Citation 10

https://www.sciencedirect.com/science/article/pii/S0024320522009833

Citation 11

https://www.hindawi.com/journals/omcl/2021/7382900/

Citation 12

https://www.sciencedirect.com/science/article/pii/S0147651321004371

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat FSH concentrations in serum, plasma and other biological fluids.

Available Sizes

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