Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit

Product Specifications

Abbreviation

NOS3; eNOS

UniProt

Q62600

Cross Reactivity

This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed

Target

ECNOS; NOS III; E-NOS

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS3/eNOS. You can calculate the concentration of Rat NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S075333222300118X

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332221008003

Citation 03

https://www.sciencedirect.com/science/article/pii/S0753332221007605

Citation 04

https://www.sciencedirect.com/science/article/pii/S0024320522002600

Citation 05

https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s12986-021-00573-0

Citation 06

https://www.sciencedirect.com/science/article/pii/S0014299921003988

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jdi.13690

Citation 08

https://www.mdpi.com/1937476

Citation 09

https://www.hindawi.com/journals/cdtp/2020/1936406/

Citation 10

https://link.springer.com/article/10.1007/s11033-019-04730-w

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066271/

Citation 12

https://www.tandfonline.com/doi/abs/10.1080/2000656X.2019.1582422

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat NOS3/eNOS concentrations in serum, plasma and other biological fluids.

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