Rat Cys-C (Cystatin C) ELISA Kit

Product Specifications

Abbreviation

Cys-C

UniProt

P14841

Cross Reactivity

This kit recognizes Rat Cys-C in samples. No significant cross-reactivity or interference between Rat Cys-C and analogues was observed

Target

CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Stem cells; Tags and Cell Markers

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Cys-C. You can calculate the concentration of Rat Cys-C in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2001037020304761

Citation 02

https://www.sciencedirect.com/science/article/pii/S0223523421002373

Citation 03

https://www.sciencedirect.com/science/article/pii/S0024320522003782

Citation 04

https://www.nature.com/articles/s41420-022-00822-y

Citation 05

https://www.sciencedirect.com/science/article/pii/S1382668922001004

Citation 06

https://www.mdpi.com/2227-9059/10/7/1613

Citation 07

https://www.karger.com/Article/FullText/452561

Citation 08

https://www.dovepress.com/alpha-chymotrypsin-protects-against-acute-lung-kidney-and-liver-injuri-peer-reviewed-fulltext-article-DDDT

Citation 09

https://www.sciencedirect.com/science/article/pii/S0378874122002604

Citation 10

https://academic.oup.com/toxres/advance-article-abstract/doi/10.1093/toxres/tfac009/6561811

Citation 11

https://academic.oup.com/jpp/advance-article-abstract/doi/10.1093/jpp/rgab091/6318411

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/fcp.12718

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat Cys-C concentrations in serum, plasma and other biological fluids.

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