Rat CRH (Corticotropin Releasing Hormone) ELISA Kit

Product Specifications

Abbreviation

CRH

UniProt

P01143

Cross Reactivity

This kit recognizes Rat CRH in samples.No significant cross-reactivity or interference between Rat CRH and analogues was observed

Target

CRF; Corticoliberin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in the sample or standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CRH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0269749122020589

Citation 02

https://www.sciencedirect.com/science/article/pii/S004896972100262X

Citation 03

https://www.frontiersin.org/articles/10.3389/fcimb.2022.945851/full?utm_source=dlvr.it&utm_medium=twitter

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/nmo.13556

Citation 05

https://www.sciencedirect.com/science/article/pii/S2405844019366630

Citation 06

http://dmd.aspetjournals.org/content/46/6/786.abstract

Citation 07

https://www.sciencedirect.com/science/article/pii/S0006291X23000773

Citation 08

https://www.tandfonline.com/doi/abs/10.1080/13880209.2020.1812674

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CRH concentrations in serum, plasma and other biological fluids.

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