Rat CASP3 (Caspase 3) ELISA Kit

Product Specifications

Abbreviation

CASP3

UniProt

P55213

Cross Reactivity

This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed

Target

CPP32; CPP32B; SCA-1; Apoptain; Yama

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP3. You can calculate the concentration of Rat CASP3 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332222009222

Citation 02

https://www.sciencedirect.com/science/article/pii/S0753332222004450

Citation 03

https://www.mdpi.com/1422-0067/22/14/7483

Citation 04

https://www.sciencedirect.com/science/article/pii/S1382668922002046

Citation 05

https://www.sciencedirect.com/science/article/pii/S0269749119337479

Citation 06

https://www.tandfonline.com/doi/abs/10.1080/13510002.2022.2129192

Citation 07

https://www.frontiersin.org/articles/10.3389/fphar.2020.610331/full

Citation 08

https://www.sciencedirect.com/science/article/pii/S027869152200669X

Citation 09

https://www.mdpi.com/1950756

Citation 10

https://www.sciencedirect.com/science/article/pii/S0009279721002209

Citation 11

https://www.sciencedirect.com/science/article/pii/S0009279723000662

Citation 12

https://www.sciencedirect.com/science/article/pii/S0024320522003095

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CASP3 concentrations in serum, plasma and other biological fluids.

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