Rat CGRP1 (Calcitonin Gene Related Peptide 1) ELISA Kit

Product Specifications

Abbreviation

CGRP1

UniProt

P01256

Cross Reactivity

This kit recognizes Rat CGRP1 in samples. No significant cross-reactivity or interference between Rat CGRP1 and analogues was observed

Target

CGRP1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Metabolism; Signal transduction; Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CGRP1. You can calculate the concentration of Rat CGRP1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1550413122004909

Citation 02

https://www.nature.com/nm/journal/v22/n10/full/nm.4162.html

Citation 03

https://www.sciencedirect.com/science/article/pii/S0944711321004153

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185139/

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1111/ejn.14897

Citation 06

https://link.springer.com/article/10.1007/s12020-018-1640-2

Citation 07

https://www.sciencedirect.com/science/article/pii/S0006899319304202

Citation 08

https://link.springer.com/article/10.1007/s10753-019-01115-w

Citation 09

https://cmjournal.biomedcentral.com/articles/10.1186/s13020-020-00386-y

Citation 10

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5765728/

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.12915

Citation 12

https://www.spandidos-publications.com/10.3892/etm.2019.8201

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat CGRP1 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

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