Rat BNP (Brain Natriuretic Peptide) ELISA Kit

Product Specifications

Abbreviation

BNP

UniProt

P13205

Cross Reactivity

This kit recognizes Rat BNP in samples.No significant cross-reactivity or interference between Rat BNP and analogues was observed

Target

NPPB; GC-B; B-Type Natriuretic Peptide; Natriuretic Peptide Precursor B

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of Rat BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.mdpi.com/1930052

Citation 02

https://www.sciencedirect.com/science/article/pii/S0024320522008232

Citation 03

https://www.mdpi.com/1103930

Citation 04

https://www.mdpi.com/1464438

Citation 05

https://www.karger.com/Article/Abstract/489653

Citation 06

https://www.frontiersin.org/articles/10.3389/fphar.2021.741623/full

Citation 07

https://europepmc.org/articles/pmc9445158/bin/table_1.doc?javascript_support=no

Citation 08

https://europepmc.org/article/pmc/pmc8678489?javascript_support=no

Citation 09

https://www.frontiersin.org/articles/10.3389/fphar.2020.00094/full

Citation 10

https://link.springer.com/article/10.1007/s12012-021-09665-y

Citation 11

https://www.sciencedirect.com/science/article/pii/S0014299920308189

Citation 12

https://www.mdpi.com/2025904

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat BNP concentrations in serum, plasma and other biological fluids.

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