Rat BAX (Bcl-2 Associated X Protein) ELISA Kit

Product Specifications

Abbreviation

BAX

UniProt

Q63690

Cross Reactivity

This kit recognizes Rat BAX in samples. No significant cross-reactivity or interference between Rat BAX and analogues was observed

Target

BCL2L4

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BAX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BAX. You can calculate the concentration of Rat BAX in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8722537/

Citation 02

https://www.sciencedirect.com/science/article/pii/S0048969720314820

Citation 03

https://www.sciencedirect.com/science/article/pii/S0024320522006397

Citation 04

https://link.springer.com/article/10.1007/s11356-023-25917-6

Citation 05

https://www.mdpi.com/1950756

Citation 06

https://www.sciencedirect.com/science/article/pii/S0300483X21003188

Citation 07

https://www.sciencedirect.com/science/article/pii/S1319562X21005830

Citation 08

https://www.mdpi.com/1473434

Citation 09

https://link.springer.com/article/10.1007/s11064-022-03640-x

Citation 10

https://link.springer.com/content/pdf/10.1007/s12640-016-9658-9.pdf

Citation 11

https://www.sciencedirect.com/science/article/pii/S0161813X18302043

Citation 12

https://link.springer.com/content/pdf/10.1007/s11011-020-00551-0.pdf

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat BAX concentrations in serum, plasma and other biological fluids.

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