Rat MMP-13 (Matrix Metalloproteinase 13) ELISA Kit

Product Specifications

Abbreviation

MMP-13

UniProt

P23097

Cross Reactivity

This kit recognizes Rat MMP-13 in samples. No significant cross-reactivity or interference between Rat MMP-13 and analogues was observed

Target

MMP13; CLG3; MANDP1; Collagenase 3

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-13. You can calculate the concentration of Rat MMP-13 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s12276-022-00879-w

Citation 02

https://www.nature.com/articles/s41598-022-09380-w

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.5949

Citation 04

https://www.tandfonline.com/doi/abs/10.1080/03008207.2021.1982915

Citation 05

https://www.tandfonline.com/doi/abs/10.1080/03008207.2022.2051499

Citation 06

https://www.hindawi.com/journals/bmri/2022/4117520/

Citation 07

https://link.springer.com/article/10.1007/s10787-017-0432-2

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807197/

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.6366

Citation 10

https://www.spandidos-publications.com/etm/10/1/97?text=fulltext

Citation 11

https://link.springer.com/content/pdf/10.1007/s10067-020-04958-z.pdf

Citation 12

https://link.springer.com/article/10.1007/s12038-019-9907-1

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat MMP-13 concentrations in serum, plasma and other biological fluids.

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