Rat MAU (Microalbuminuria) ELISA Kit

Product Specifications

Abbreviation

MAU

UniProt

P02770

Cross Reactivity

This kit recognizes Rat MAU in samples. No significant cross-reactivity or interference between Rat MAU and analogues was observed

Target

UMA

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MAU. You can calculate the concentration of Rat MAU in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 μg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 μg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332222013361

Citation 02

https://www.tandfonline.com/doi/abs/10.1080/13813455.2021.1874996

Citation 03

https://www.sciencedirect.com/science/article/pii/S0944711319302260

Citation 04

https://academic.oup.com/toxres/advance-article-abstract/doi/10.1093/toxres/tfac009/6561811

Citation 05

https://www.sciencedirect.com/science/article/pii/S0006291X22014760

Citation 06

https://www.spandidos-publications.com/etm/15/2/2156

Citation 07

https://www.jstage.jst.go.jp/article/bpb/41/8/41_b17-00930/_article/-char/ja/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat MAU concentrations in serum, plasma and other biological fluids.

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