Rat IL-4 (Interleukin 4) ELISA Kit

Product Specifications

Abbreviation

IL-4

UniProt

P20096

Cross Reactivity

This kit recognizes Rat IL-4 in samples. No significant cross-reactivity or interference between Rat IL-4 and analogues was observed

Target

IL4; BCGF-1; BCGF1; BSF-1; BSF1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-4. You can calculate the concentration of Rat IL-4 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201680

Citation 02

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01277-0

Citation 03

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12168

Citation 04

https://www.sciencedirect.com/science/article/pii/S0944711321004153

Citation 05

https://www.frontiersin.org/articles/10.3389/fcimb.2021.783049/full?utm_source=dlvr.it&utm_medium=twitter

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874122010601

Citation 07

https://www.mdpi.com/1420-3049/27/13/4256

Citation 08

https://link.springer.com/article/10.1007/s10787-021-00832-9

Citation 09

https://www.hindawi.com/journals/mi/2020/4260204/

Citation 10

https://www.sciencedirect.com/science/article/pii/S037887412100790X

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1111/jgh.15987

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.26009

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat IL-4 concentrations in serum, plasma and other biological fluids.

Available Sizes

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