Rat IFN-γ (Interferon Gamma) ELISA Kit

Product Specifications

Abbreviation

IFN-γ

UniProt

P01581

Cross Reactivity

This kit recognizes Rat IFN-γ in samples. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed

Target

IFNG; IFG; IFI; Type II Interferon

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvac181/6872578

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202201680

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7778587/

Citation 04

https://link.springer.com/content/pdf/10.1007/s00018-016-2426-4.pdf

Citation 05

https://www.sciencedirect.com/science/article/pii/S0944711321004153

Citation 06

https://www.frontiersin.org/articles/10.3389/fimmu.2020.01611/full?report=reader

Citation 07

https://www.sciencedirect.com/science/article/pii/S0024320521009449

Citation 08

https://www.sciencedirect.com/science/article/pii/S0378874122010601

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300736/

Citation 10

https://link.springer.com/article/10.1007/s10753-022-01763-5

Citation 11

https://link.springer.com/article/10.1007/s00213-021-05941-5

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/tox.23464

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.

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