Rat EPO (Erythropoietin) ELISA Kit

Product Specifications

Abbreviation

EPO

UniProt

P29676

Cross Reactivity

This kit recognizes Rat EPO in samples. No significant cross-reactivity or interference between Rat EPO and analogues was observed

Target

EP; MVCD2; Epoetin; Hematopoietin; Hemopoietin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EPO. You can calculate the concentration of Rat EPO in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

62.5-4000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

37.5 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://portlandpress.com/clinsci/article-abstract/135/19/2285/229841

Citation 02

https://www.mdpi.com/1422-0067/20/20/4985

Citation 03

https://www.sciencedirect.com/science/article/pii/S0162013420302002

Citation 04

https://www.hindawi.com/journals/jdr/2019/6753541/abs/

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8542477/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874117321463

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332219307577

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257330/

Species

Rat

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Rat EPO concentrations in serum, plasma and other biological fluids.

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