Porcine TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Product Specifications

Abbreviation

TNF-α

UniProt

P23563

Cross Reactivity

This kit recognizes Porcine TNF-α in samples. No significant cross-reactivity or interference between Porcine TNF-α and analogues was observed

Target

DIF; TNF-alpha; TNFA; TNFSF2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine TNF-α. You can calculate the concentration of Porcine TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-022-03752-6

Citation 02

https://www.sciencedirect.com/science/article/pii/S2405654522000142

Citation 03

https://www.sciencedirect.com/science/article/pii/S2405654521001530

Citation 04

https://jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00492-9

Citation 05

https://www.mdpi.com/1420-3049/24/7/1220

Citation 06

https://www.worldscientific.com/doi/abs/10.1142/S0192415X2050007X

Citation 07

https://iopscience.iop.org/article/10.1088/1748-605X/ac1d3e/meta

Citation 08

https://www.mdpi.com/2076-2615/9/10/738

Species

Porcine

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Porcine TNF-α concentrations in Serum, plasma and other biological fluids.

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