Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

Product Specifications

Abbreviation

TNF-α

UniProt

P06804

Cross Reactivity

This kit recognizes Mouse TNF-α in samples.No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed

Target

DIF; TNF-alpha; TNFA; TNFSF2; TNF; TNFa

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Immunology; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41556-022-00853-8

Citation 02

https://www.sciencedirect.com/science/article/pii/S0889159122004445

Citation 03

https://pubs.acs.org/doi/abs/10.1021/acscentsci.2c01424

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.202001771

Citation 05

https://www.sciencedirect.com/science/article/pii/S1385894722059460

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202105391

Citation 07

https://www.sciencedirect.com/science/article/pii/S1385894722054195

Citation 08

https://www.sciencedirect.com/science/article/pii/S1385894722060995

Citation 09

https://www.science.org/doi/abs/10.1126/scitranslmed.aaz6667

Citation 10

https://www.sciencedirect.com/science/article/pii/S0142961222003301

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202207350

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202204388

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-α concentrations in serum, plasma and other biological fluids.

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