Mouse IFNα (Interferon Alpha) ELISA Kit

Product Specifications

Abbreviation

IFNα

UniProt

P01572, P01573

Cross Reactivity

This kit recognizes Mouse IFNα in samples.No significant cross-reactivity or interference between Mouse IFNα and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Immunology; Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFNα. You can calculate the concentration of Mouse IFNα in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.28203

Citation 02

https://www.sciencedirect.com/science/article/pii/S1742706121005018

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982817/

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/imm.13379

Citation 05

https://www.sciencedirect.com/science/article/pii/S0223523421008205

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13904

Citation 07

https://academic.oup.com/endo/article-abstract/162/12/bqab180/6359040

Citation 08

https://pubs.rsc.org/lv/content/articlehtml/2018/fo/c7fo01161j

Citation 09

https://link.springer.com/article/10.1186/s12865-022-00515-2

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.14174

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IFNα concentrations in serum, plasma and other biological fluids.

Available Sizes

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