Mouse LH (Luteinizing Hormone) ELISA Kit

Product Specifications

Abbreviation

UniProt

O09108

Cross Reactivity

This kit recognizes Mouse LH in samples.No significant cross-reactivity or interference between Mouse LH and analogues was observed

Target

Lutropin; Lutrophin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LH. You can calculate the concentration of Mouse LH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1186/s12989-022-00453-2

Citation 02

https://www.sciencedirect.com/science/article/pii/S0269749121011398

Citation 03

https://www.sciencedirect.com/science/article/pii/S0891584922009455

Citation 04

https://europepmc.org/article/pmc/pmc9027572?javascript_support=no

Citation 05

https://diabetes.diabetesjournals.org/content/early/2020/11/27/db20-0436.abstract

Citation 06

https://www.sciencedirect.com/science/article/pii/S0048969720345782

Citation 07

https://europepmc.org/article/pmc/pmc9691951?javascript_support=no

Citation 08

https://www.sciencedirect.com/science/article/pii/S0026049519300356

Citation 09

https://www.sciencedirect.com/science/article/pii/S0753332222004504

Citation 10

https://www.dovepress.com/pseudoephedrine-nanoparticles-alleviate-adriamycin-induced-reproductiv-peer-reviewed-fulltext-article-IJN

Citation 11

https://www.nature.com/articles/s42003-019-0552-x

Citation 12

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo04239d

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse LH concentrations in serum, plasma and other biological fluids.

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