Mouse Cys-C (Cystatin C) ELISA Kit

Product Specifications

Abbreviation

Cys-C

UniProt

P21460

Cross Reactivity

This kit recognizes Mouse Cys-C in samples.No significant cross-reactivity or interference between Mouse Cys-C and analogues was observed

Target

CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Cardiovascular; Stem cells; Tags and Cell Markers

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Cys-C. You can calculate the concentration of Mouse Cys-C in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S2213231719313850

Citation 02

https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-022-03629-8

Citation 03

https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-022-03717-9

Citation 04

https://www.sciencedirect.com/science/article/pii/S1931524422000020

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7955787/

Citation 06

https://www.sciencedirect.com/science/article/pii/S1567576921001508

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332220309914

Citation 08

https://www.sciencedirect.com/science/article/pii/S0196978121001352

Citation 09

https://www.sciencedirect.com/science/article/pii/S0378427420303544

Citation 10

https://www.physiology.org/doi/abs/10.1152/ajprenal.00405.2018

Citation 11

https://www.karger.com/Article/FullText/492589

Citation 12

https://www.biomedcentral.com/openurl?doi=10.1186/s11671-016-1424-7

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Cys-C concentrations in serum, plasma and other biological fluids.

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