Mouse CTXI (Cross Linked C-telopeptide of Type I Collagen) ELISA Kit

Product Specifications

Abbreviation

CTXI

UniProt

P11087

Cross Reactivity

This kit recognizes Mouse CTXI in samples.No significant cross-reactivity or interference between Mouse CTXI and analogues was observed

Target

CTX-I; CTX1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Metabolism; Cardiovascular; Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTXI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTXI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTXI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CTXI. You can calculate the concentration of Mouse CTXI in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202100808

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202004831

Citation 03

https://www.nature.com/articles/s41418-020-00731-6

Citation 04

https://www.nature.com/articles/s41467-022-29191-x

Citation 05

https://www.embopress.org/doi/abs/10.15252/emmm.202115373

Citation 06

https://www.sciencedirect.com/science/article/pii/S1534580722006839

Citation 07

https://www.nature.com/articles/s41413-019-0056-9

Citation 08

https://www.nature.com/articles/s41418-022-01068-y

Citation 09

https://www.nature.com/articles/s41413-018-0012-0

Citation 10

https://www.sciencedirect.com/science/article/pii/S2213231721003402

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086346/

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019162/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse CTXI concentrations in serum, plasma and other biological fluids.

Available Sizes

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