Mouse AMH (Anti-Mullerian Hormone) ELISA Kit

Product Specifications

Abbreviation

AMH

UniProt

P27106

Cross Reactivity

This kit recognizes Mouse AMH in samples.No significant cross-reactivity or interference between Mouse AMH and analogues was observed

Target

MIF; MIH; MIS

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AMH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AMH. You can calculate the concentration of Mouse AMH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.78-50 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.47 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0160412020322534

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/acel.12662

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381702/

Citation 04

https://www.sciencedirect.com/science/article/pii/S2352396418300458

Citation 05

https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac097/6625847

Citation 06

https://link.springer.com/article/10.1186/s12958-022-00956-9

Citation 07

https://academic.oup.com/biolreprod/article-abstract/104/6/1347/6161978

Citation 08

https://www.sciencedirect.com/science/article/pii/S0378874121002233

Citation 09

https://www.nature.com/articles/s41598-020-63948-y

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1111/aji.13686

Citation 11

https://www.hindawi.com/journals/acp/2021/1781532/

Citation 12

https://www.sciencedirect.com/science/article/pii/S1642431X22000377

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse AMH concentrations in serum, plasma and other biological fluids.

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