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Mouse Aβ1-40 (Amyloid Beta 1-40) ELISA Kit

Product Specifications

Abbreviation

Aβ1-40

UniProt

P12023

Cross Reactivity

This kit recognizes Mouse Aβ1-40 in samples.No significant cross-reactivity or interference between Mouse Aβ1-40 and analogues was observed

Target

Aβ40; Amyloid Beta 40

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Aβ1-40. You can calculate the concentration of Mouse Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/loadet

Product MSDS

https://789.bio/eb/COWXr1

Citation 01

https://www.tandfonline.com/doi/abs/10.1080/15548627.2019.1596488

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.14102

Citation 03

https://link.springer.com/article/10.1007/s13311-022-01204-z

Citation 04

https://www.mdpi.com/1883524

Citation 05

https://www.mdpi.com/2072-6643/14/15/3179

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12874

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.7619

Citation 08

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8828541/

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993748/

Citation 10

https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo02651h

Citation 11

https://www.sciencedirect.com/science/article/pii/S0944711322001921

Citation 12

https://www.sciencedirect.com/science/article/pii/S1756464622002845

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Aβ1-40 concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

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