Mouse INS (Insulin) ELISA Kit

Product Specifications

Abbreviation

INS

UniProt

P01325, P01326

Cross Reactivity

This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed

Target

IDDM2; ILPR; IRDN; MODY10

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Cell culture supernatant and tissue homogenate

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41419-021-04160-2

Citation 02

https://www.sciencedirect.com/science/article/pii/S0963996922012650

Citation 03

https://www.sciencedirect.com/science/article/pii/S1043661818301683

Citation 04

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.0c01467

Citation 05

https://www.sciencedirect.com/science/article/pii/S0014482719303076

Citation 06

https://link.springer.com/article/10.1007/s11356-018-1337-3

Citation 07

https://www.spandidos-publications.com/10.3892/mmr.2022.12686

Citation 08

https://www.hindawi.com/journals/ecam/2021/5514175/

Citation 09

https://journals.sagepub.com/doi/abs/10.1177/1535370221997780

Citation 10

https://link.springer.com/article/10.1007/s00795-019-00216-7

Citation 11

https://link.springer.com/article/10.1007/s40200-022-01135-8

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in cell culture supernatant and tissue homogenate.

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