Mouse Ang-II (Angiotensin II) ELISA Kit

Product Specifications

Abbreviation

Ang-II

UniProt

P11859

Cross Reactivity

This kit recognizes Mouse Ang-II in samples.No significant cross-reactivity or interference between Mouse Ang-II and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Ang-II. During the reaction, Mouse Ang-II in the sample or standard competes with a fixed amount of Mouse Ang-II on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse Ang-II. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Ang-II in tested samples can be calculated by comparing the OD of the samples to the standard curve.