Mouse INS (Insulin) ELISA Kit

Product Specifications

Abbreviation

INS

UniProt

P01325, P01326

Cross Reactivity

This kit recognizes Mouse INS in samples.No significant cross-reactivity or interference between Mouse INS and analogues was observed

Target

IDDM2; ILPR; IRDN; MODY10

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.13-200 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

1.88 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41467-022-35481-1

Citation 02

https://www.nature.com/articles/s41413-018-0012-0

Citation 03

https://link.springer.com/article/10.1007/s00018-022-04306-1

Citation 04

https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.2c02003

Citation 05

https://www.mdpi.com/1920772

Citation 06

https://www.sciencedirect.com/science/article/pii/S0963996922012650

Citation 07

https://www.sciencedirect.com/science/article/pii/S002432052300125X

Citation 08

https://www.hindawi.com/journals/omcl/2020/6538930/

Citation 09

https://pubs.rsc.org/en/content/articlelanding/2023/fo/d2fo03687h

Citation 10

https://www.sciencedirect.com/science/article/pii/S075333222200110X

Citation 11

https://www.hindawi.com/journals/omcl/2021/4706410/

Citation 12

https://www.sciencedirect.com/science/article/pii/S0891584918314394

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

NBP3-15953-100ul 100 µL

Rabbit Polyclonal BRD4 Antibody

Ask

View Details

TP318354L 1 mg

PIK3C2B (NM_002646) Human Recombinant Protein

Ask

View Details

MBS8119964-01 0.1 mg

TrkC Protein, Human, Recombinant (His & hFc Tag)

Ask

View Details

MBS8119964-02 5x 0.1 mg

TrkC Protein, Human, Recombinant (His & hFc Tag)

Ask

View Details

MBS8248575-01 0.01 mg

Recombinant Human ATF1 Protein

Ask

View Details

MBS8248575-02 0.05 mg

Recombinant Human ATF1 Protein

Ask

View Details