Mouse VIP (Vasoactive Intestinal Peptide) ELISA Kit
Product Specifications
Abbreviation
VIP
UniProt
P32648
Cross Reactivity
This kit recognizes Mouse VIP in samples.No significant cross-reactivity or interference between Mouse VIP and analogues was observed
Target
PHM27
Type
Competitive-ELISA
Applications
ELISA
Field of Research
Cancer; Neuroscience
Assay Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse VIP. During the reaction, Mouse VIP in the sample or standard competes with a fixed amount of Mouse VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse VIP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Performance Time
2 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.5-800 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
7.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Citation 01
https://www.mdpi.com/2072-6643/14/19/4083
Citation 02
https://link.springer.com/article/10.1186/s13023-022-02528-x
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse VIP concentrations in Serum, plasma and other biological fluids.
Available Sizes
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