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Mouse TXB2 (Thromboxane B2) ELISA Kit

Product Specifications

Abbreviation

TXB2

Cross Reactivity

This kit recognizes Mouse TXB2 in samples.No significant cross-reactivity or interference between Mouse TXB2 and analogues was observed

Target

TX-B2

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Metabolism; Immunology

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse TXB2. During the reaction, Mouse TXB2 in the sample or standard competes with a fixed amount of Mouse TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse TXB2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse TXB2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Product Datasheet

https://789.bio/ea/g8y5ql

Product MSDS

https://789.bio/eb/Om9ezT

Citation 01

https://www.atsjournals.org/doi/abs/10.1164/rccm.201406-1031OC

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 03

https://www.frontiersin.org/articles/10.3389/fimmu.2018.01948/full

Citation 04

https://www.mdpi.com/1422-0067/22/18/9915

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.8b00781

Citation 06

https://www.spandidos-publications.com/10.3892/mmr.2017.6969?text=abstract

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TXB2 concentrations in serum, plasma and other biological fluids.

Available Sizes

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