Mouse MT (Melatonin) ELISA Kit

Product Specifications

Abbreviation

Cross Reactivity

This kit recognizes Mouse MT in samples.No significant cross-reactivity or interference between Mouse MT and analogues was observed

Target

N-Acetyl-5-Methoxytryptamine

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse MT. During the reaction, Mouse MT in the sample or standard competes with a fixed amount of Mouse MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

7.81-500 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

4.69 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12573

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12851

Citation 03

https://www.pnas.org/content/115/29/E6880.short

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359097/

Citation 05

https://www.mdpi.com/2073-4409/11/10/1690

Citation 06

https://www.sciencedirect.com/science/article/pii/S0006295222001678

Citation 07

https://www.sciencedirect.com/science/article/pii/S0378517321003896

Citation 08

https://link.springer.com/article/10.1007/s40618-023-02014-6

Citation 09

https://www.mdpi.com/2079-7737/11/7/962

Citation 10

https://www.sciencedirect.com/science/article/pii/S1050464821000644

Citation 11

https://www.nature.com/articles/s41598-020-59568-1

Citation 12

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877689/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse MT concentrations in serum, plasma and other biological fluids.

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