Mouse IL-18 (Interleukin 18) ELISA Kit

Product Specifications

Abbreviation

IL-18

UniProt

P70380

Cross Reactivity

This kit recognizes Mouse IL-18 in samples. No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed

Target

IL18; IGIF; IL-1g; IL-1F4; Interferon-gamma-inducing factor

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-18. You can calculate the concentration of Mouse IL-18 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/art.42420

Citation 02

https://onlinelibrary.wiley.com/doi/abs/10.1111/jpi.12855

Citation 03

https://www.sciencedirect.com/science/article/pii/S2095809922006804

Citation 04

https://bpspubs.onlinelibrary.wiley.com/doi/abs/10.1111/bph.16061

Citation 05

https://www.sciencedirect.com/science/article/pii/S1742706121005018

Citation 06

https://www.nature.com/articles/s41419-020-2565-2

Citation 07

https://www.sciencedirect.com/science/article/pii/S2352345X22002387

Citation 08

https://www.mdpi.com/1941176

Citation 09

https://www.sciencedirect.com/science/article/pii/S1043661821000669

Citation 10

https://link.springer.com/article/10.1007/s10565-023-09790-0

Citation 11

https://www.sciencedirect.com/science/article/pii/S094471132200441X

Citation 12

https://www.sciencedirect.com/science/article/pii/S0944711322004159

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.

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