Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) ELISA Kit

Product Specifications

Abbreviation

NOS2; iNOS

UniProt

P29477

Cross Reactivity

This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed

Target

NOS2A; HEP-NOS; I-NOS

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.31-20 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.19 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://europepmc.org/article/med/36800290?javascript_support=no

Citation 02

https://www.sciencedirect.com/science/article/pii/S0022202X21012379

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0141813023001058

Citation 05

https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00527a

Citation 06

https://www.sciencedirect.com/science/article/pii/S0753332223000525

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.24109

Citation 08

https://www.sciencedirect.com/science/article/pii/S075333222201277X

Citation 09

https://pubs.acs.org/doi/abs/10.1021/acs.biomac.1c00425

Citation 10

https://www.sciencedirect.com/science/article/pii/S1549963421001040

Citation 11

https://www.sciencedirect.com/science/article/pii/S0308814620311031

Citation 12

https://cmbl.biomedcentral.com/articles/10.1186/s11658-018-0107-z

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse NOS2/iNOS concentrations in serum, plasma and other biological fluids.

Available Sizes

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