Mouse HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Product Specifications

Abbreviation

HIF-1α

UniProt

Q61221

Cross Reactivity

This kit recognizes Mouse HIF-1α in samples. No significant cross-reactivity or interference between Mouse HIF-1α and analogues was observed

Target

HIF1A; HIF-1A; MOP1; PASD8; bHLHe78; basic helix-loop-helix transcription factor

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HIF-1α. You can calculate the concentration of Mouse HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.202202625

Citation 02

https://www.sciencedirect.com/science/article/pii/S0022202X21012379

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055295/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332218386372

Citation 05

https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.1c00647

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874121004177

Citation 07

https://stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1031-x

Citation 08

https://www.karger.com/Article/Abstract/444810

Citation 09

https://link.springer.com/article/10.1007/s10753-018-0815-6

Citation 10

https://www.sciencedirect.com/science/article/pii/S0895398819300455

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse HIF-1α concentrations in serum, plasma and other biological fluids.

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