Mouse HMGB-1 (High Mobility Group Protein B1) ELISA Kit

Product Specifications

Abbreviation

HMGB-1

UniProt

P63158

Cross Reactivity

This kit recognizes Mouse HMGB-1 in samples. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed

Target

HMGB1; HMG1; HMG3; SBP-1; Amphoterin

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Epigenetics and Nuclear Signaling

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201905213

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acsnano.0c09681

Citation 03

https://www.sciencedirect.com/science/article/pii/S0142961222005427

Citation 04

https://www.sciencedirect.com/science/article/pii/S0142961222004811

Citation 05

https://www.nature.com/articles/s41467-021-24564-0

Citation 06

https://www.sciencedirect.com/science/article/pii/S2452199X21005946

Citation 07

https://www.sciencedirect.com/science/article/pii/S0142961221004877

Citation 08

https://www.sciencedirect.com/science/article/pii/S0142961221006682

Citation 09

https://www.sciencedirect.com/science/article/pii/S2211383521004366

Citation 10

https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202004905

Citation 11

https://immunology.sciencemag.org/content/4/36/eaau6426.abstract

Citation 12

https://www.sciencedirect.com/science/article/pii/S1043661823000361

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse HMGB-1 concentrations in serum, plasma and other biological fluids.

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