Mouse Hepc (Hepcidin) ELISA Kit

Product Specifications

Abbreviation

Hepc

UniProt

Q9EQ21

Cross Reactivity

This kit recognizes Mouse Hepc in samples. No significant cross-reactivity or interference between Mouse Hepc and analogues was observed

Target

Hepcidin Antimicrobial Peptide

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Hepc. You can calculate the concentration of Mouse Hepc in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0278691521000077

Citation 02

https://www.mdpi.com/2076-3921/10/10/1590

Citation 03

https://www.mdpi.com/1422-0067/23/7/3593

Citation 04

https://www.frontiersin.org/articles/10.3389/fimmu.2018.00860/full

Citation 05

https://www.mdpi.com/2076-3921/9/6/514

Citation 06

https://www.frontiersin.org/articles/10.3389/fimmu.2019.00131/full

Citation 07

https://www.mdpi.com/834890

Citation 08

https://www.frontiersin.org/articles/10.3389/fphys.2022.897179/full?utm_source=dlvr.it&utm_medium=twitter

Citation 09

https://jme.bioscientifica.com/view/journals/jme/60/4/JME-17-0301.xml

Citation 10

https://www.sciencedirect.com/science/article/pii/S0946672X21000869

Citation 11

https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26715

Citation 12

https://www.sciencedirect.com/science/article/pii/S0006291X19310113

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse Hepc concentrations in serum, plasma and other biological fluids.

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