Mouse GC (Glucagon) ELISA Kit

Product Specifications

Abbreviation

UniProt

P55095

Cross Reactivity

This kit recognizes Mouse GC in samples.No significant cross-reactivity or interference between Mouse GC and analogues was observed

Target

GCG; GLP1; GLP2; GRPP

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Developmental biology; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GC. During the reaction, Mouse GC in the sample or standard competes with a fixed amount of Mouse GC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse GC in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0092867422015379

Citation 02

https://diabetes.diabetesjournals.org/content/66/2/287.abstract

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051025/

Citation 04

https://www.sciencedirect.com/science/article/pii/S1756464622003930

Citation 05

https://academic.oup.com/endo/advance-article-abstract/doi/10.1210/endocr/bqac217/6967064

Citation 06

https://www.sciencedirect.com/science/article/pii/S0143417920301281

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse GC concentrations in serum, plasma and other biological fluids.

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