Mouse FSH (Follicle Stimulating Hormone) ELISA Kit

Product Specifications

Abbreviation

FSH

UniProt

Q60687 (FSHB)

Cross Reactivity

This kit recognizes Mouse FSH in samples. No significant cross-reactivity or interference between Mouse FSH and analogues was observed

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FSH. You can calculate the concentration of Mouse FSH in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.56-100 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://link.springer.com/article/10.1186/s12989-022-00453-2

Citation 02

https://www.nature.com/articles/s41419-021-04277-4

Citation 03

https://www.sciencedirect.com/science/article/pii/S0269749121011398

Citation 04

https://www.sciencedirect.com/science/article/pii/S0891584922009455

Citation 05

https://europepmc.org/article/pmc/pmc9691951?javascript_support=no

Citation 06

https://www.sciencedirect.com/science/article/pii/S0026049519300356

Citation 07

https://www.nature.com/articles/s42003-019-0552-x

Citation 08

https://www.nature.com/articles/s41419-019-1455-y

Citation 09

https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo04239d

Citation 10

https://www.nature.com/articles/srep21776/

Citation 11

https://link.springer.com/article/10.1186/s12958-022-00956-9

Citation 12

https://rbej.biomedcentral.com/articles/10.1186/s12958-021-00702-7

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse FSH concentrations in serum, plasma and other biological fluids.

Available Sizes

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