Mouse D2D (D-Dimer) ELISA Kit

Product Specifications

Abbreviation

D2D

Cross Reactivity

This kit recognizes Mouse D2D in samples.No significant cross-reactivity or interference between Mouse D2D and analogues was observed

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cardiovascular

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse D2D. During the reaction, Mouse D2D in the sample or standard competes with a fixed amount of Mouse D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse D2D. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse D2D in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Plasma

Detection Range

78.13-5000 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

46.88 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41589-022-01095-3

Citation 02

https://www.pnas.org/content/117/51/32648.short

Citation 03

https://www.sciencedirect.com/science/article/pii/S014296121630120X

Citation 04

https://www.sciencedirect.com/science/article/pii/S2473952922003111

Citation 05

https://www.sciencedirect.com/science/article/pii/S0006295219302886

Citation 06

https://www.frontiersin.org/articles/10.3389/fimmu.2019.01073/full

Citation 07

https://www.sciencedirect.com/science/article/pii/S0006291X23001031

Citation 08

https://www.sciencedirect.com/science/article/pii/S0895398819300455

Citation 09

https://link.springer.com/article/10.1186/s40635-021-00428-1

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse D2D concentrations in plasma.

Available Sizes

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