Mouse CASP3 (Caspase 3) ELISA Kit

Product Specifications

Abbreviation

CASP3

UniProt

P70677

Cross Reactivity

This kit recognizes Mouse CASP3 in samples. No significant cross-reactivity or interference between Mouse CASP3 and analogues was observed

Target

CPP32; CPP32B; SCA-1; Apoptain; Yama

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cell Biology; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP3. You can calculate the concentration of Mouse CASP3 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332221012403

Citation 02

https://www.sciencedirect.com/science/article/pii/S0161813X21000322

Citation 03

https://www.tandfonline.com/doi/abs/10.1080/09603123.2022.2105824

Citation 04

https://www.frontiersin.org/articles/10.3389/fphys.2020.00962/full

Citation 05

https://onlinelibrary.wiley.com/doi/abs/10.1002/brb3.2778

Citation 06

https://www.sciencedirect.com/science/article/pii/S0306452222001324

Citation 07

https://www.mdpi.com/2075-4426/12/12/2012

Citation 08

https://journals.sagepub.com/doi/abs/10.1177/0960327117703689

Citation 09

https://www.sciencedirect.com/science/article/pii/S2214750022000841

Citation 10

https://www.tandfonline.com/doi/abs/10.1080/0972060X.2019.1625814

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse CASP3 concentrations in serum, plasma and other biological fluids.

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