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Mouse BDNF (Brain Derived Neurotrophic Factor) ELISA Kit

Product Specifications

Abbreviation

BDNF

UniProt

P21237

Cross Reactivity

This kit recognizes Mouse BDNF in samples. No significant cross-reactivity or interference between Mouse BDNF and analogues was observed

Target

ANON2; BULN2

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse BDNF. You can calculate the concentration of Mouse BDNF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://europepmc.org/articles/pmc9530393/bin/datasheet_1.pdf?javascript_support=no

Citation 02

https://www.mdpi.com/1422-0067/23/17/10184

Citation 03

https://link.springer.com/article/10.1007/s13311-022-01339-z

Citation 04

https://www.mdpi.com/1104266

Citation 05

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009530/

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874122010637

Citation 07

https://www.sciencedirect.com/science/article/pii/S0028390822002283

Citation 08

https://www.sciencedirect.com/science/article/pii/S027869152030048X

Citation 09

https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7840451/

Citation 10

https://www.sciencedirect.com/science/article/pii/S0161813X21000322

Citation 11

https://www.sciencedirect.com/science/article/pii/S0014299922000590

Citation 12

https://link.springer.com/article/10.1007/s11064-022-03672-3

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse BDNF concentrations in serum, plasma and other biological fluids.

Available Sizes

Frequently Asked Questions

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