Mouse AST (Aspartate Aminotransferase) ELISA Kit

Product Specifications

Abbreviation

AST

UniProt

P05201

Cross Reactivity

This kit recognizes Mouse AST in samples. No significant cross-reactivity or interference between Mouse AST and analogues was observed

Target

SGOT; serum glutamic oxaloacetic transaminase; GOT1; AspAT; ASAT; AAT

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Signal transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AST. You can calculate the concentration of Mouse AST in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.16-10 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.1 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://journals.asm.org/doi/abs/10.1128/mBio.01155-21

Citation 02

https://www.mdpi.com/2040816

Citation 03

https://www.mdpi.com/1342984

Citation 04

https://www.sciencedirect.com/science/article/pii/S016201342100266X

Citation 05

https://www.sciencedirect.com/science/article/pii/S1756464621002309

Citation 06

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Citation 10

https://pubmed.ncbi.nlm.nih.gov/33015817/

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