Mouse ACTH (Adrenocorticotropic Hormone) ELISA Kit

Product Specifications

Abbreviation

ACTH

UniProt

P01193

Cross Reactivity

This kit recognizes Mouse ACTH in samples.No significant cross-reactivity or interference between Mouse ACTH and analogues was observed

Target

Corticotropin; POMC; Adrenocorticotropin

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse ACTH. During the reaction, Mouse ACTH in the sample or standard competes with a fixed amount of Mouse ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/cjoc.202000652

Citation 02

https://pubs.rsc.org/en/content/articlelanding/2021/fo/d1fo01608c

Citation 03

https://www.sciencedirect.com/science/article/pii/S1756464622003334

Citation 04

https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202101570R

Citation 05

https://www.sciencedirect.com/science/article/pii/S0944711320302671

Citation 06

https://www.nature.com/articles/s41598-021-82604-7

Citation 07

https://www.nature.com/articles/s41598-020-66379-x

Citation 08

https://www.sciencedirect.com/science/article/pii/S0018506X17303343

Citation 09

https://www.sciencedirect.com/science/article/pii/S0166432817312160

Citation 10

https://pubmed.ncbi.nlm.nih.gov/30683828/

Citation 11

https://www.sciencedirect.com/science/article/pii/S0034528819303431

Citation 12

https://link.springer.com/article/10.1007/s41105-018-0166-6

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse ACTH concentrations in serum, plasma and other biological fluids.

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