Mouse CRP (C-Reactive Protein) ELISA Kit

Product Specifications

Abbreviation

CRP

UniProt

P14847

Cross Reactivity

This kit recognizes Mouse CRP in samples.No significant cross-reactivity or interference between Mouse CRP and analogues was observed

Target

PTX1; Pentraxin 1

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Epigenetics and Nuclear Signaling; Metabolism; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CRP. You can calculate the concentration of Mouse CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.03-25 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.02 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1385894722054195

Citation 02

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051948/

Citation 03

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358619/

Citation 04

https://www.sciencedirect.com/science/article/pii/S0753332221012403

Citation 05

https://www.sciencedirect.com/science/article/pii/S0753332221009173

Citation 06

https://www.sciencedirect.com/science/article/pii/S0378874122010996

Citation 07

https://www.sciencedirect.com/science/article/pii/S0753332220309495

Citation 08

https://link.springer.com/article/10.1007/s10753-021-01506-y

Citation 09

https://journals.lww.com/shockjournal/FullText/2018/04000/Protection_Against_Lipopolysaccharide_Induced.15.aspx

Citation 10

https://www.sciencedirect.com/science/article/pii/S075333221935351X

Citation 11

https://www.mdpi.com/1153336

Citation 12

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185688

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse CRP concentrations in serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MR207875 10 µg

Cyp2a12 (NM_133657) Mouse Tagged ORF Clone

Ask

View Details

46306141 1x 10^6 Cells / 1.0 mL

TBX22 CRISPR Knock Out 293T Cell Line (Human)

Ask

View Details

MBS9395757-01 48 Well

Camel Netrin-5 (NTN5) ELISA Kit

Ask

View Details

MBS9395757-02 96 Well

Camel Netrin-5 (NTN5) ELISA Kit

Ask

View Details

MBS9395757-03 5x 96 Well

Camel Netrin-5 (NTN5) ELISA Kit

Ask

View Details

MBS9395757-04 10x 96 Well

Camel Netrin-5 (NTN5) ELISA Kit

Ask

View Details