Mouse TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit

Product Specifications

Abbreviation

TGF-β1

UniProt

P04202

Cross Reactivity

This kit recognizes Mouse TGF-β1 in samples.No significant cross-reactivity or interference between Mouse TGF-β1 and analogues was observed

Target

TGF-B1; TGFB; TGFbeta; CED; DPD1; LAP

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Metabolism; Signal transduction; Stem cells

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TGF-β1. You can calculate the concentration of Mouse TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

31.25-2000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

18.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://pubs.acs.org/doi/abs/10.1021/acsami.2c14448

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acsami.2c13976

Citation 03

https://www.sciencedirect.com/science/article/pii/S0168365921002996

Citation 04

https://europepmc.org/articles/pmc9530393/bin/datasheet_1.pdf?javascript_support=no

Citation 05

https://pubmed.ncbi.nlm.nih.gov/35860242/

Citation 06

https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13316

Citation 07

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587338/

Citation 08

https://www.sciencedirect.com/science/article/pii/S0045653520323730

Citation 09

https://pubs.rsc.org/en/content/articlelanding/2022/tb/d2tb00527a

Citation 10

https://www.sciencedirect.com/science/article/pii/S0308814621025954

Citation 11

https://www.sciencedirect.com/science/article/pii/S0147651320302037

Citation 12

https://www.hindawi.com/journals/omcl/2022/5249367/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TGF-β1 concentrations in Serum, plasma and other biological fluids.

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

RG226145 10 µg

STRIP2 (NM_001134336) Human Tagged ORF Clone

Ask

View Details

CRJ0121-01 15 nmol

ROBO4 siRNA (Human)

Ask

View Details

CRJ0121-02 30 nmol

ROBO4 siRNA (Human)

Ask

View Details

SR317764 1 Kit

Polyserase1 (TMPRSS9) Human siRNA Oligo Duplex (Locus ID 360200)

Ask

View Details

MC202640 10 µg

Ythdf1 (NM_173761) Mouse Untagged Clone

Ask

View Details

MBS9218232-01 0.5 mg

IGF2 Antibody (Center R54) Blocking peptide

Ask

View Details