Mouse IL-17A (Interleukin 17A) ELISA Kit

Product Specifications

Abbreviation

IL-17A

UniProt

Q62386

Cross Reactivity

This kit recognizes Mouse IL-17A in samples. No significant cross-reactivity or interference between Mouse IL-17A and analogues was observed

Target

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-17A. You can calculate the concentration of Mouse IL-17A in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.nature.com/articles/s41536-022-00258-z

Citation 02

https://pubmed.ncbi.nlm.nih.gov/35860242/

Citation 03

https://www.sciencedirect.com/science/article/pii/S0022202X21012379

Citation 04

https://www.nature.com/articles/s41419-020-02857-4?elqTrackId=53a15fcae36543ceb5431d3cbd6ac94b

Citation 05

https://www.sciencedirect.com/science/article/pii/S0045653520323730

Citation 06

https://europepmc.org/article/pmc/pmc9178196?javascript_support=no

Citation 07

https://www.hindawi.com/journals/omcl/2023/2894695/

Citation 08

https://www.nature.com/articles/s41401-020-00580-6

Citation 09

https://www.mdpi.com/1422-0067/23/1/226

Citation 10

https://www.sciencedirect.com/science/article/pii/S1567576922004842

Citation 11

https://www.mdpi.com/2072-6643/13/9/3209

Citation 12

https://www.hindawi.com/journals/sci/2022/5522828/

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IL-17A concentrations in serum, plasma and other biological fluids.

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