Mouse IFN-β (Interferon Beta) ELISA Kit

Product Specifications

Abbreviation

IFN-β

UniProt

P01575

Cross Reactivity

This kit recognizes Mouse IFN-β in samples. No significant cross-reactivity or interference between Mouse IFN-β and analogues was observed

Target

IFNB1; IFB; IFF; IFNB

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cancer; Cardiovascular; Immunology

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-β. You can calculate the concentration of Mouse IFN-β in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.28203

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acsnano.2c11764

Citation 03

https://www.nature.com/articles/s41564-019-0428-3

Citation 04

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330518/

Citation 05

https://www.sciencedirect.com/science/article/pii/S2213231722001392

Citation 06

https://link.springer.com/article/10.1007/s12274-022-5102-z

Citation 07

https://europepmc.org/article/med/36800290?javascript_support=no

Citation 08

https://www.sciencedirect.com/science/article/pii/S1742706121005018

Citation 09

https://www.sciencedirect.com/science/article/pii/S0141813022027842

Citation 10

https://www.sciencedirect.com/science/article/pii/S0141813022014738

Citation 11

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982817/

Citation 12

https://onlinelibrary.wiley.com/doi/abs/10.1111/imm.13315

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-β concentrations in serum, plasma and other biological fluids.

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